Incipient cytorrhysis assay
Web(A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying concentrations (1-4 M). WebJan 26, 2016 · Appressorium turgor was measured by incipient cytorrhysis assay using a 2- to 5 M glycerol solution as previously described (Foster et al. 2003). The onion penetration assay was performed as previously described (Wang et al. 2013). Onion epidermal strips were isolated and 40 μl of conidial suspension was inoculated onto the adaxial surface.
Incipient cytorrhysis assay
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WebAug 29, 2016 · Appressoria formed at 24 h post-incubation were used in an incipient cytorrhysis assay to measure appressorial turgor. The experiments were replicated three times and more than 200 appressoria were counted for … WebFeb 18, 2024 · Considering that the germinating and appressorial formation were normal in the Mohat1 mutant (Table S1), we measured the internal concentration of solute and the turgor pressure within appressoria by the incipient cytorrhysis (cell collapse) assay using 1–4 molar of glycerol [ 45 ].
WebIn an incipient cytorrhysis (cell collapse) assay using a 1–4 molar concentration of glycerol solution, appressoria of the ΔMomip11 mutant showed increased collapse rates in … WebAug 4, 2014 · (A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying …
WebNational Center for Biotechnology Information WebSep 6, 2012 · Appressorial turgor pressure was determined by incipient-cytorrhysis assay reported previously ( Howard et al. 1991 ). The mycelia were placed onto artificial cellophane maintained on water agar plates and incubated at 25 °C, and the samples were microscopically observed at intervals.
WebOct 3, 2024 · Patients with collagen vascular diseases, hepatic cirrhosis, schistosomiasis, and other parasitic infections can produce false-positive results. There is a strong cross …
WebNov 16, 2024 · An incipient cytorrhysis assay was carried out by allowing appressoria to form in water on borosilicate cover slips for 24 hr, after which the water was replaced with a range of aqueous glycerol ranging from 0.25 to 2.5M and, after 30 min, the frequency of cytorrhysis determined ( de Jong et al. 1997 ). duck season victoria 2022 datesWebIncipient cytorrhysis determined the turgor pressure in appressoria of P. pachyrhizi to be equivalent to 5.13 MPa. MZM data revealed that osmotically active sugar alcohols only … duck season in ontarioWebDec 20, 2011 · The turgor pressure exerted by a mature appressorium was measured using an incipient cytorrhysis test reported earlier ( Howard et al. 1991 ). The rate of appressoria formation on a hydrophobic surface was similar in both the wild-type strain Guy11 and the Moplaa deletion mutant, as shown in Table 2. commonwealth courts portal user guideWebApr 1, 2002 · CYP1 putatively encodes a mitochondrial and cytosolic form of cyclophilin, and targeted gene replacement has shown that CYP1 acts as a virulence determinant in rice blast. Cyp1 mutants show reduced virulence and are impaired in associated functions, such as penetration peg formation and appressorium turgor generation. commonwealth courts portal e-file divorceWebSep 9, 2024 · For the spraying assay on rice, 2-week-old rice seedlings (n ≥ 17) were inoculated by spraying with 2.5 ml conidial suspensions ... Incipient cytorrhysis assays for appressorium turgor pressure (n = 3). The appressoria at 24 hpi were incubated in 0.5, 1.0, or 1.5 M of glycerol solutions, and the collapsed and plasmolyzed appressoria were ... duck season in coloradoWebNov 2, 2015 · Appressorium turgor was measured by incipient cytorrhysis assay using a 2- to 5-molar concentration of glycerol solution, as previously described (Foster et al ., 2003 ). Visualization of glycogen and lipid during appressorium development Appressorium formation was induced on the coverslips (Fisher Scientific). commonwealth court of pennsylvania docketWebApr 7, 2024 · Appressorium turgor pressure was analyzed by the incipient cytorrhysis assay using 1–4 M of glycerol solution, as described by Wang et al. (2024). The experiment was conducted three times with at least 100 structures per replicate. Onion penetration assays were conducted as described by Wang et al. (2024). duck season in kansas