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Pherd20t

WebThe sspA and sspB genes from P . aeruginosa were cloned behind the P BAD promoter in the pHERD20T vector, and conjugated into CF149. (A) Western blot analysis of SspA and … Web13. apr 2024 · .本发明属于基因突变技术领域，尤其是一种新型转座子突变株文库的构建系统、新型转座子突变文库和应用。背景技术.转座子(tn)，亦称跳跃基因，是指能将自身基因 …

CRISPR‐dCas9‐mediated knockdown of prtR, an essential gene in ...

WebCarefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a … Web6. dec 2024 · The highest PYO production was found in ΔpilA/pHerd20T, which showed low bioelectricity output (half of PAO1/pHerd20T). In ΔpilA or PAO1, expression of either … megan and tony https://dawkingsfamily.com

Viruses Free Full-Text The Odd “RB” Phage—Identification of ...

A previously described31 environmental strain of P. aeruginosa was used as a template to amplify the four cas genes of the Type I-C CRISPR–Cas system genes (cas3, cas5, cas7 and cas8). The genes were cloned into … Zobraziť viac Transformations of P. aeruginosa, E. coli, P. syringae and K. pneumoniae strains were conducted using standard electroporation … Zobraziť viac PAO1IC was grown overnight at 37 °C in LB media. Then 150 μl of overnight culture was added to 4 ml of 0.7% LB top agar and spread on 1.5% LB agar plates supplemented … Zobraziť viac The isogenic DMS3macrIIA4 and acrIC1 phages were constructed using previously described methods60. A recombination cassette, pJZ01, was constructed with homology to the … Zobraziť viac Web... pHERD20T is an arabinose-inducible vector based on pUCP20T that supports replication in P. aeruginosa and shares characteristics of the pUCP vector family. meganandty.com

Viruses Free Full-Text The Odd “RB” Phage—Identification of ...

Category:Screening for Growth-Inhibitory ORFans in - Springer

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Pherd20t

一种铜绿假单胞菌CRISPRi系统的构建方法与流程 - X技术

Web7. okt 2024 · The resultant pHERD20T-rpoS plasmid was used as template to amplify a 2376 bp DNA fragment with the oligonucleotides araCTHindIIIFw and rpoSHindIIIRv. The PCR … WebThe key point is that the dcas9 gene is located downstream of the P BAD promoter. pHERD20T is an arabinose-inducible vector based on pUCP20T that supports replication …

Pherd20t

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Web26. nov 2024 · Additionally, overproduction of Pf4r via pHERD20T-pf4r in the PAO1 wild-type strain reduced the ability of Pf4 to re-infect PAO1 approximately 100-fold, and … WebNational Center for Biotechnology Information

Web10. mar 2024 · bioRxiv.org - the preprint server for Biology Web16. dec 2012 · The pHERD20T vector was digested with EcoRI and HindIII and then infused using the IN-Fusion EcoDry cloning kit (Clontech) following the manufacturer’s instructions.

Web22. aug 2013 · Strains containing pHERD20T-algU WT (PAO1), pHERD20T-algU A61V (CF149), and pHERD20T were streaked on PIA plates supplemented with 300 µg/ml of … WebEscherichia-Pseudomonas shuttle vector pHERD20T: Escherichia-Pseudomonas shuttle vector pHERD26T: Escherichia-Pseudomonas shuttle vector pHERD28T: Escherichia …

Web5. sep 2024 · A recombination cassette consisting of genes 34 to 38 of phage JBD30 (anti-CRISPR locus with large flanking regions) in plasmid pHERD20T was previously generated …

WebPlasmid pUCP20T-ecfp from Dr. Mariette Barbier's lab contains the insert eCFP and is published in PLoS One. 2016 Mar 3;11(3):e0146827. doi: 10.1371/journal.pone.0146827. … megan and tylerWebpHERD20T [20], used for the construction of antisense libraries (see below), are E. coli/Pseudomonas shuttle vectors carrying araC/ P BAD pair and ampicillin/carbenicillin … nam joo hyuk fashion weightlifting fairyWeb4. apr 2024 · Pf prophages are ssDNA filamentous prophages that are prevalent among various Pseudomonas aeruginosa strains. The genomes of Pf prophages contain not only … megan and wes babyWebPhage lysates were serially diluted, and 10 μl samples were spotted on lawns of strain ΔPf4 carrying pHERD20T or pHERD20T-pf4r. Discussion In this study, we provided evidences … nam joo-hyuk three meals a day in gochangWebpet15b,pet-15b载体价格800元，载体质粒图谱(Vector map),载体序列(Sequence)和多克隆位点见下文，抗性为Ampicillin，大小为5708bp，测序引物为T7和T7t，含有N-His标签和N … megan and toryWebpet15b载体简介. pET-15b载体具有N端His标签和thrombin酶切位点，用于周质腔蛋白表达。. 独特的酶切位点在载体图谱中被标注出来。. 需要注意的是，这些载体序列是依据载 … megan and travisWeb15. jún 2024 · Pseudomonas aeruginosa (PA) is a major human pathogen for hospital-acquired infections. We report the genetic code expansion of this opportunistic pathogen … nam joo hyuk scarlet heart